Vie cataboliche e anaboliche

13 MATERIALI E METODI Ceppi utilizzati Streptomyces coelicolor - M145 (wild type) (SCP1 - SCP2 - ) Kieser et al., (2000). - kynu - M145/pIJ kynu /pij kynu/pijkynu Micrococcus luteus wild type Escherichia coli - BW25113/pIJ790 Plasmide: pij790 [orir101], [repa101(ts)], arabp-gam-bet-exo Cromosoma: ( (arad-arab)567, lacz4787(::rrnb-4), lacip-4000(laciq), -, rpos369(am), rph-1, (rhad-rhab)568, hsdr514;(datsenko and Wanner, 2000). - BW25141/pKD78 Plasmide: pkd78 [orir101], [repa101(ts)], arabp-gam-bet-exo Cromosoma: (laciq rrnbt14 DlacZWJ16 DphoBR580 hsdr514 DaraBADAH33 DrhaBADLD78 galu95 end- ABT333 uida(dmlui)::pir1 reca1) - DH5 /pij773 Plasmide: pbluescript KS (+),aac(3)iv, orit (RK2), FRT sites Cromosoma: (F-Φ80dlacZΔM15Δ(lacZY A-argF)U169 enda1 reca1 hdsr17 deor thi-1 supe44 λ- gyra96 rel1a1) - ET12567/pUZ8002 Plasmide: Paget et al., 1999 Cromosoma: (F- dam-13::tn9 dcm- 6hsdM hsd R zjj -202::Tn10 recf143 galk2 galt22 ara14 lacy1 xyl- 5 leub6) - DH5 F -, ø80dlaczδm15, Δ(lacZYA-argF)U169, deor, reca1, enda1, hsdr17(rk -, mk + ), phoa, supe44, λ -, thi- 1, gyra96, rela1. - TOP10: F - mcra Δ(mrr-hsdRMS-mcrBC) φ80laczδm15 ΔlacX74 reca1 arad139 Δ(araleu)7697 galu galk rpsl (StrR) enda1 nupg - DH10B: F - mcra Δ(mrr-hsdRMS-mcrBC) Φ80lacZ ΔM15 ΔlacX74 reca1 arad139 (ara-leu)7967 galu galk λ - rpsl nupg tona - BL21(DE3) plys F - dcm ompt hsds(r B m B ) gal λ(de3) [plyss Cam r ] - BL21(DE3)pLysS/pRSETB, BL21(DE3)pLysS/pRSETB-SCO3644, BL21(DE3)pLysS/pRSETB-SCO3645, BL21(DE3)pLysS/pRSETB-SCO

Vie cataboliche e anaboliche

vie cataboliche e anaboliche

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